MersV1, Main, Exploration, bibRecord, 004F66

Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Identifieur interne : 004F66 ( Main/Exploration ); précédent : 004F65; suivant : 004F67

Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Auteurs : Max D. Summers [États-Unis] ; Pepper Hoops [États-Unis]

Source :

Virology [ 0042-6822 ] ; 1980.

RBID : ISTEX:7C4E286BD3F79303C61798D0AA6D5597EBDBEA73

English descriptors

Teeft :
- Acmnpv, Acmnpv polyhedrin, Antigenic, Antigenic determinants, Antiserum, Assay, Baculovirus, Baculoviruses, Cell lysates, Determinant, Dicapua, Final concentration, Granulin, Granulin proteins, Granulins, Immunodiffusion, Immunological, Immunological analysis, Immunoreactivity, Nuclear polyhedrosis viruses, Peptide, Polyhedrin, Polyhedrin proteins, Polyhedrins, Polyhedron, Polyhedrosis, Protease, Radioimmunoassay, Room temperature, Sfgv, Sfgv granulin, Tngv, Tngv granulin, Tngv granulin antiserum, Tnsnpv polyhedrin, Trichoplusia, Tris buffer, Tryptic, Tryptic peptides, Viral.

Abstract

Abstract: Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.

Url:

https://api.istex.fr/ark:/67375/6H6-M9D4D045-L/fulltext.pdf

DOI: 10.1016/0042-6822(80)90128-2

Affiliations:

États-Unis

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Le document en format XML

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<term>Antigenic determinants</term>
<term>Antiserum</term>
<term>Assay</term>
<term>Baculovirus</term>
<term>Baculoviruses</term>
<term>Cell lysates</term>
<term>Determinant</term>
<term>Dicapua</term>
<term>Final concentration</term>
<term>Granulin</term>
<term>Granulin proteins</term>
<term>Granulins</term>
<term>Immunodiffusion</term>
<term>Immunological</term>
<term>Immunological analysis</term>
<term>Immunoreactivity</term>
<term>Nuclear polyhedrosis viruses</term>
<term>Peptide</term>
<term>Polyhedrin</term>
<term>Polyhedrin proteins</term>
<term>Polyhedrins</term>
<term>Polyhedron</term>
<term>Polyhedrosis</term>
<term>Protease</term>
<term>Radioimmunoassay</term>
<term>Room temperature</term>
<term>Sfgv</term>
<term>Sfgv granulin</term>
<term>Tngv</term>
<term>Tngv granulin</term>
<term>Tngv granulin antiserum</term>
<term>Tnsnpv polyhedrin</term>
<term>Trichoplusia</term>
<term>Tris buffer</term>
<term>Tryptic</term>
<term>Tryptic peptides</term>
<term>Viral</term>
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<front><div type="abstract" xml:lang="en">Abstract: Granulin and polyhedrin proteins were purified by preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis from the baculoviruses Autographa californica, Rachiplusia ou, Heliothis zea, Heliothis armigera, Trichoplusia ni, and Spodoptera frugiperda. Antisera were raised against Autographa californica (Ac) polyhedrin and Trichoplusia ni (Tn) granulin and analyzed for homologous and heterologous immunoreactivity by immunodiffusion and radioimmunoassay (RIA). Ac polyhedrin and Tn granulin antisera recognized antigenic determinants on several baculovirus polyhedrin and granulin proteins even though the heterologous proteins had different immunoreactivities when compared by competition radioimmunoassay. Antigenic differences among granulin and polyhedrin proteins were also detected by altered slopes of the competition reaction curves. Antiserum raised against Ac polyhedrin which was purified in the presence of SDS was tested by competition RIA for its ability to detect and react with native polyhedrin produced in the infected TN-368 cells. Ac polyhedrin antiserum had similar if not identical ability to bind to native polyhedrin and to polyhedrin purified in the presence of SDS.</div>
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Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri